Genifal Herpes (GH) is herpes simplex that occurs on the genital area. It is a viral infectious skin disease. Traditional Chinese medicine belongs to the category of hot sores. Unlike herpes simplex, which occurs outside the mouth of the mouth, it is mostly transmitted through sexual relations. So, what are the methods to check for genital herpes?
1。cytological examination
After taking samples, smeared directly and subjected to Wright's staining, Giemsa's staining or Papanicolaou's staining to help diagnosis when eosinophilic inclusion bodies are found in the nuclei of multinucleated giant cells.
The sensitivity of this method is only 50% to 80%, and it is not specific. The positive rate is high in the early stage of the disease and low in the later stage of the disease.
2。etiological examination
(1) HSV examination: Direct immunofluorescence test using fluorescein-labeled anti-HSV-1 and anti-HSV-2 antibodies can distinguish the types of HSV.
(2) HSV antigen test: Indirect immunofluorescence test, enzyme-linked immunosorbent test
vibrator for vaginal atrophy , Western blot test or radioimmunoassay can also be used to detect HSV antigen.
(3) HSV nucleic acid testing: It includes nucleic acid probe testing and nucleic acid amplification testing. The latter is divided into PCR and LCR. It has strong sensitivity and specificity, is fast, simple, and has low requirements on detection materials.
(4) HSV culture and isolation: Commonly used cell culture methods are mainly used for further identification of HSV.
(5) Electron microscope examination: Blister fluid or diseased brain tissue was taken for slices, and virus particles were observed under electron microscope. The positive rate was 50%. However
bullet vibrator , HSV and other herpes viruses were difficult to distinguish in morphology, so immunoelectron microscope examination was more specific.
3。serological examination
It is mainly used to detect anti-HSV-1 and anti-HSV-2 antibodies, diagnose primary infection of HSV
realistic dildo , and conduct seroepidemiological investigation of HSV infection. Immunofluorescence test, enzyme-linked immunosorbent test, Western blot test and radioimmunoassay can be used to detect. In modern times
big dildo , HSV-2 gD2 glycoprotein is used as antigen to detect anti-HSV-2 antibodies, and HSV-1 gDl or gC glycoprotein is used as antigen to detect anti-HSV-1 antibodies. The sensitivity is high and anti-HSV-1 and anti-HSV-2 antibodies can be distinguished. However, Such tests can only indicate that the patient has had an explicit or invisible HSV infection.
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